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McDonald JC (1985) Inorganic Chromatographic Analy- sis. 2 gave the mechanistic rate quoted as d12Etotal 14 k112Eactual12OHactual þ k212HEþactual12OHactual dt The observed rate quoted was ð8:17Þ ð8:18Þ ð8:20Þ ð8:21Þ giving d12Etotal 14 kobs12Etotal12OHactual dt kobs12Etotal 14 k112Eactual þ k212HEþactual k 14 k 12Eactual þ k 12HEþactual obs 112E 212E total total Since Kb is small, it was possible to approximate [E]actual 14 [E]total to give the simple relationship k 14k þk Kb ð8:26Þ obs 1 2 12OHactual The approximation made for the glycine ethyl ester may not be justified in situations where there is a significant amount of all species involved in the equilibrium present, and a more rigorous analysis must be used.

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Enhanced selectivity of enzymes by kinetic proofreading. In contrast, if a test is used to confirm a diagnosis that is likely to be treated with an invasive procedure. It was not until 1910 that J. 5percentand109percentwhen4animalsperdose are used, - 93 per cent and 108 per cent when 6 animals per dose are used.

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